Carbohydrate ligands for endothelial - Leukocyte adhesion molecule 1

Journal Article · Fri Feb 15 04:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.4.1138· OSTI ID:6398103

Tiemeyer, M; Swiedler, S J; Ishihara, Masayuki; Moreland, M; Schweingruber, H; Hirtzer, P; Brandley, B K ^[1]

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1. Glycomed, Inc., Alameda, CA (United States)

The acute inflammatory response requires that circulating leukocytes bind to and penetrate the vascular wall to access the site of injury. Several receptors have been implicated in this interaction, including a family of putative carbohydrate-binding proteins. The authors report here the identification of an endogenous carbohydrate ligand for one of these receptors, endothelial-leukocyte adhesion molecule 1 (ELAM-1). Radiolabeled COS cells transfected with a plasmid containing the cDNA for ELAM-1 were used as probes to screen glycolipids extracted from human leukocytes. COS cells transfected with this plasmid adhered to a subset of sialylated glycolipids resolved on TLC plates or adsorbed on polyvinyl chloride microtiter wells. Adhesion to these glycolipids required calcium but was not inhibited by heparin, chondroitin sulfate, keratan sulfate, or yeast phosphomannan. Monosaccharide composition, linkage analysis, and fast atom bombardment mass spectrometry of the glycolipids indicate that the ligands for ELAM-1 are terminally sialylated lactosylceramides with a variable number of N-acetyllactosamine repeats and at least one fucosylated N-acetylglucosamine residue.

• OSTI ID: 6398103
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (United States) Vol. 88:4; ISSN PNASA; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADHESION
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CHEMISTRY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ENDOTHELIUM
GLYCOLIPIDS
INFLAMMATION
INJURIES
ISOTOPES
LECTINS
LEUKOCYTES
LIGANDS
LIGHT NUCLEI
LIPIDS
MATERIALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SACCHARIDES
SEPARATION PROCESSES
SYMPTOMS
THIN-LAYER CHROMATOGRAPHY
TISSUES