Identification of a carbohydrate-based endothelial ligand for a lymphocyte homing receptor
Journal Article
·
· Journal of Cell Biology; (United States)
- Department of Anatomy, University of California, San Francisco (USA)
Lymphocyte attachment to high endothelial venules within lymph nodes is mediated by the peripheral lymph node homing receptor (pnHR), originally defined on mouse lymphocytes by the MEL-14 mAb. The pnHR is a calcium-dependent lectin-like receptor, a member of the LEC-CAM family of adhesion proteins. Here, using a soluble recombinant form of the homing receptor, we have identified an endothelial ligand for the pnHR as an {approximately} 50-kD sulfated, fucosylated, and sialylated glycoprotein, which we designate Sgp50 (sulfated glycoprotein of 50 kD). Recombinant receptor binding to this lymph node-specific glycoprotein requires calcium and is inhibitable by specific carbohydrates and by MEL-14 mAb. Sialylation of the component is required for binding. Additionally, the glycoprotein is precipitated by MECA-79, an adhesion-blocking mAb reactive with lymph node HEV. A related glycoprotein of {approximately} 90 kD (designated as Sgp90) is also identified.
- OSTI ID:
- 5398647
- Journal Information:
- Journal of Cell Biology; (United States), Journal Name: Journal of Cell Biology; (United States) Vol. 113:5; ISSN 0021-9525; ISSN JCLBA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADHESION
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CHEMICAL COMPOSITION
CONNECTIVE TISSUE CELLS
ENDOTHELIUM
GLYCOPROTEINS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
LIGANDS
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICE
MOLECULAR WEIGHT
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
REACTION KINETICS
RECEPTORS
RODENTS
SOMATIC CELLS
SULFATES
SULFUR COMPOUNDS
SULFUR ISOTOPES
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ADHESION
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBOHYDRATES
CHEMICAL COMPOSITION
CONNECTIVE TISSUE CELLS
ENDOTHELIUM
GLYCOPROTEINS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
LIGANDS
LYMPH NODES
LYMPHATIC SYSTEM
LYMPHOCYTES
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MICE
MOLECULAR WEIGHT
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PROTEINS
REACTION KINETICS
RECEPTORS
RODENTS
SOMATIC CELLS
SULFATES
SULFUR COMPOUNDS
SULFUR ISOTOPES
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES