Heterogeneity of basal keratinocytes: nonrandom distribution of thymidine-labeled basal cells in confluent cultures is not a technical artifact
Journal Article
·
· J. Invest. Dermatol.; (United States)
Basal surface autoradiography of (/sup 3/H)dThd-labeled, confluent, keratinocyte cultures reveals that proliferating cells have a nonrandom, patterned distribution. Unlabeled cells, likewise, appear nonrandomly in clusters. The authors show here that failure to detect DNA synthesis in some basal cells in culture is not an artifact caused either by physical separation of the labeled nuclei from the radiographic emulsion or by a diffusion barrier that would prevent (/sup 3/H)dThd from reaching basal cells.
- OSTI ID:
- 6380774
- Journal Information:
- J. Invest. Dermatol.; (United States), Journal Name: J. Invest. Dermatol.; (United States) Vol. 6; ISSN JIDEA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
AUTORADIOGRAPHY
AZINES
BODY
CELL CONSTITUENTS
CELL CULTURES
CELL NUCLEI
CELL PROLIFERATION
DIFFUSION
DNA
EPIDERMIS
EPITHELIUM
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
KERATIN
LABELLED COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
RIBOSIDES
SCLEROPROTEINS
SKIN
THYMIDINE
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMAL TISSUES
AUTORADIOGRAPHY
AZINES
BODY
CELL CONSTITUENTS
CELL CULTURES
CELL NUCLEI
CELL PROLIFERATION
DIFFUSION
DNA
EPIDERMIS
EPITHELIUM
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
KERATIN
LABELLED COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
RIBOSIDES
SCLEROPROTEINS
SKIN
THYMIDINE
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS