Thin-layer mapping of peptides labeled with /sup 3/H or /sup 14/C via reductive methylation

Journal Article · Sun Apr 01 04:00:00 EST 1979 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(79)90804-2· OSTI ID:6372398

Nelles, L P; Bamburg, J R

Two dimensional tryptic peptide maps have been obtained from 2 ..mu..g (40 pmol) of protein digest following labeling with /sup 3/H or /sup 14/C via reductive methylation. A simple labeling procedure is complete within 1 h; autoradiographs of /sup 14/C-labeled maps and fluorographs of /sup 3/H-labeled maps are obtained in 72 and 24 h, respectively. Tryptic peptide maps of /sup 14/C//sup 3/H methylated ..cap alpha..- and ..beta..-tubulin, and rabbit muscle, chick muscle, and chick brain actins show approximately the expected number of peptides. Methylation does not appear to measurably alter the map positions of the peptides relative to unmethylated peptides in the solvent systems used for either electrophoresis or chromatography.

• Research Organization: Colorado State Univ., Fort Collins
• OSTI ID: 6372398
• Journal Information: Anal. Biochem.; (United States), Journal Name: Anal. Biochem.; (United States) Vol. 94:1; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBON 14
CARBON ISOTOPES
CHEMICAL PROPERTIES
CHEMICAL REACTIONS
EVEN-EVEN NUCLEI
HYDROGEN ISOTOPES
ISOTOPES
LABELLING
LIGHT NUCLEI
METHYLATION
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDES
PROTEINS
RADIOISOTOPES
TISSUES
TRITIUM
YEARS LIVING RADIOISOTOPES