Analytical peptide mapping by high performance liquid chromatography
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:5266473
Peptide mapping of underivatized tryptic digests of bovine and chick intestinal calcium-binding proteins has been accomplished by high performance liquid chromatography (HPLC). High precision analysis of nanomolar quantities of peptides were achieved in less than 1 h (recycle time). Peak resolution and definition are superior compared to conventioal techniques and recoveries of both small (4-residue) hydrophilic and large (30-residue) hydrophobic peptides are excellent. The total amino acid composition of the bovine intestinal calcium-binding protein has been accounted for on the basis of two tryptic maps of 20 ..mu..g of protein each.
- Research Organization:
- New York State Coll. of Veterinary Medicine, Ithaca, NY
- DOE Contract Number:
- EY-76-S-02-2792
- OSTI ID:
- 5266473
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 254:15; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
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OSTI ID:6080173
Related Subjects
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
AMINO ACIDS
ANIMALS
BIRDS
BODY
CALCIUM
CARBOXYLIC ACIDS
CATTLE
CHICKENS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ELEMENTS
FOWL
GASTROINTESTINAL TRACT
INTESTINES
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAPPING
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
RUMINANTS
SEPARATION PROCESSES
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METALS
AMINO ACIDS
ANIMALS
BIRDS
BODY
CALCIUM
CARBOXYLIC ACIDS
CATTLE
CHICKENS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
DOMESTIC ANIMALS
ELEMENTS
FOWL
GASTROINTESTINAL TRACT
INTESTINES
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAPPING
METALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
RUMINANTS
SEPARATION PROCESSES
VERTEBRATES