Retroviral and psuedogene insertion sites reveal the lineage of human salivary and pancreatic amylase genes from a single gene during primate evolution

Journal Article · Fri Jun 01 04:00:00 EDT 1990 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.10.6.2513· OSTI ID:6369756

Samuelson, L C; Snow, C M; Meisler, M H ^[1]; Wiebauer, K ^[2]

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1. Michigan Univ., Ann Arbor, MI (USA). Dept. of Human Genetics
2. Friedrich Meischer Inst., CH-4002 Basel (CH)

The authors have analyzed the junction regions of inserted elements within the human amylase gene complex. This complex contains five genes which are expressed at high levels either in the pancreas or in the parotid gland. The proximal 5{prime}-flanking regions of these genes contain two inserted elements. A {gamma}-actin pseudogene is located at a position 20 base pairs upstream of the first coding exon. All of the amylase genes contain this insert. The subsequent insertion of an endogenous retrovirus interrupted the {gamma}-actin pseudogene within its 3{prime}-untranslated region. Nucleotide sequence analysis of the inserted elements associated with each of the five human amylase genes has revealed a series of molecular events during the recent history of this gene family. The data indicate that the entire gene family was generated during primate evolution from one ancestral gene copy and that the retroviral insertion activated a cryptic promoter.

• OSTI ID: 6369756
• Journal Information: Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 10:6; ISSN 0270-7306; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ACTIN
AMYLASE
BODY
DIGESTIVE SYSTEM
DNA SEQUENCING
ENDOCRINE GLANDS
ENZYMES
GENE AMPLIFICATION
GENE RECOMBINATION PROTEINS
GENE REGULATION
GENE REPRESSORS
GENES
GENETIC ENGINEERING
GLANDS
GLYCOSYL HYDROLASES
HYDROLASES
MICROORGANISMS
MOLECULAR BIOLOGY
NUCLEOPROTEINS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANS
PANCREAS
PARASITES
PROTEINS
RFLPS
SALIVARY GLANDS
STRUCTURAL CHEMICAL ANALYSIS
VIRUSES