Simultaneous 3-methylcholanthrene and antioxidant induction enhance the expression of rat liver p-nitrophenol UDP-glucuronyltransferase
To elucidate the gene source of the UDP-glucuronyltransferase (UDP-GT) family of enzymes, the authors have investigated the effects on the induction of p-nitrophenol UDP-GT by a combined treatment of rats with the carcinogen, 3-methylcholanthrene (3-MC) and the phenolic antioxidant, butylated hydroxyanisole (BHA). In this study, the elution profile of the enzyme was determined by ion exchange and affinity chromatography. P-nitrophenol UDP-GT activity was found to elute in the salt fractions of a 0-500 mM KCl gradient and required at least 5 mM UDP-glucuronic acid (UDPGA) to elute from the affinity column and has a M/sub r/ of 56,000. The results indicate that BHA + 3-MC is a more potent inducer of UDP-GT compared to either of the inducers used alone. For further investigation of the molecular basis of the enhanced induction, a cDNA library was generated from the BHA + 3-MC treated rat liver mRNA, and some mRNA from the same treatment was in vitro translated to establish the identities of the products compared to the in vivo translated proteins from the different treatments. The authors results suggest that the BHA and 3-MC induce the expression of different UDP-GT genes and the increased induction is due to these respective genes.
- Research Organization:
- Atlanta Univ., GA
- OSTI ID:
- 6366483
- Report Number(s):
- CONF-870644-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
3-METHYLCHOLANTHRENE
BIOLOGICAL EFFECTS
ANTIOXIDANTS
TRANSFERASES
ENZYME ACTIVITY
CARCINOGENS
GENES
ION EXCHANGE CHROMATOGRAPHY
LIVER
MESSENGER-RNA
RATS
ANIMALS
AROMATICS
BODY
CHROMATOGRAPHY
DIGESTIVE SYSTEM
ENZYMES
GLANDS
HYDROCARBONS
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
POLYCYCLIC AROMATIC HYDROCARBONS
RNA
RODENTS
SEPARATION PROCESSES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology