Surface morphology and morphometry of rat alveolar macrophages after ozone exposure

Journal Article · Sat Sep 01 00:00:00 EDT 1990 · Journal of Toxicology and Environmental Health; (USA)

DOI:https://doi.org/10.1080/15287399009531437· OSTI ID:6358090

Dormans, J A; Rombout, P J; van Loveren, H ^[1]

---

1. National Institute of Public Health and Environmental Protection, Bilthoven (Netherlands)

As the ultrastructural data on the effects of ozone on pulmonary alveolar macrophages (PAM) are lacking, transmission (TEM) and scanning (SEM) electron microscopy were performed on rat PAM present in alveolar lavages following exposure to ozone. Rats were continuously exposed for 7 d to ozone concentrations ranging from 0.25 to 1.50 mg/m3 for 7 d followed by a 5-d recovery period. Additionally, morphometry on lung sections was performed to quantitate PAM. In a second experiment rats were continuously exposed to 1.50 mg O3/m3 for 1, 3, 5, or 7 d. To study the influence of concurrent ozone exposure and lung infection, due to Listeria monocytogenes, rats were exposed for 7 d to 1.50 mg O3/m3 after a Listeria infection. The surface area of lavaged control PAM was uniformly covered with ruffles as shown by SEM and TEM. Exposure to 0.5 mg ozone/m3 for 7 d resulted in cells partly covered with microvilli and blebs in addition to normal ruffles. The number of large size PAM increased with an increase in ozone concentration. After 1 d of exposure, normal-appearing as well as many small macrophages with ruffles and scattered lymphocytes were seen. Lavage samples taken after 5 or 7 d of exposure showed an identical cell composition to that taken after 3 d of exposure. After Listeria infection alone, lavage samples consisted of mainly lymphocytes and some macrophages. Small quantitative changes, such as an increase in the number of polymorphonuclear neutrophils and large-size PAM, occurred in lavages after ozone exposure and infection with L. monocytogenes. Morphometric examination of lung sections revealed a concentration-related increase in the number of PAM, even in animals exposed to 0.25 mg ozone/m3 for 7 d. Centriacinar regions were more severely affected than other regions of lung tissue.

• OSTI ID: 6358090
• Journal Information: Journal of Toxicology and Environmental Health; (USA), Vol. 31:1; ISSN 0098-4108
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LUNGS
ULTRASTRUCTURAL CHANGES
OZONE
TOXICITY
CELL MEMBRANES
ERYTHROCYTES
LAVAGE
LYMPHOCYTES
LYSOSOMES
MACROPHAGES
RATS
RESPIRATORY SYSTEM DISEASES
SCANNING ELECTRON MICROSCOPY
TRANSMISSION ELECTRON MICROSCOPY
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTRON MICROSCOPY
LEUKOCYTES
MAMMALS
MATERIALS
MEMBRANES
MICROSCOPY
MORPHOLOGICAL CHANGES
ORGANOIDS
ORGANS
PHAGOCYTES
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology