Bacterial analysis of water and sediment samples
Technical Report
·
OSTI ID:6357880
Water and sediment samples collected from the Monongahela River, WV, were analyzed for total bacteria by direct counts using epifluorescence microscopy and the lipopolysaccharide (LPS) content by the limulus amebocyte lysate assay. The population ranges of bacteria in water and sediment samples were 2.4 to 6.5 x 10/sup 6//ml and 0.7 to 2 x 10/sup 10//g dry weight, respectively. The bound LPS in water samples ranges from 3 to 17 ng/ml. Sediment samples contained 17 to 34 ..mu..g of bound LPS/g dry weight of sediment. The relationship between bound LPS and bacterial cell number and the estimated bacterial carbon biomass by direct bacterial counts and LPS assay are discussed. The average ratios of the direct bacterial-count-derived carbon to the lipopolysaccharide-derived carbon for water and sediment samples were 1.03 +- 0.60 and 1.10 +- 0.53, respectively.
- Research Organization:
- Brookhaven National Lab., Upton, NY (USA)
- DOE Contract Number:
- AC02-76CH00016
- OSTI ID:
- 6357880
- Report Number(s):
- BNL-51375; ON: DE81024272
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
520200* -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
BACTERIA
BIOMASS
CARBOHYDRATES
CARBON
CENTRAL REGION
CHEMICAL ANALYSIS
DATA
ECOLOGICAL CONCENTRATION
ELEMENTS
ENERGY SOURCES
ENVIRONMENTAL QUALITY
EXPERIMENTAL DATA
HYDROGEN COMPOUNDS
INFORMATION
LIPIDS
LIPOPOLYSACCHARIDES
MICROORGANISMS
NONMETALS
NORTH AMERICA
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLLUTION
POLYSACCHARIDES
POPULATION DYNAMICS
QUANTITY RATIO
RENEWABLE ENERGY SOURCES
RIVERS
SACCHARIDES
SAMPLING
SEDIMENTS
STREAMS
SURFACE WATERS
TURBIDITY
USA
WATER
WATER POLLUTION
WATER QUALITY
WEST VIRGINIA
Aquatic-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
BACTERIA
BIOMASS
CARBOHYDRATES
CARBON
CENTRAL REGION
CHEMICAL ANALYSIS
DATA
ECOLOGICAL CONCENTRATION
ELEMENTS
ENERGY SOURCES
ENVIRONMENTAL QUALITY
EXPERIMENTAL DATA
HYDROGEN COMPOUNDS
INFORMATION
LIPIDS
LIPOPOLYSACCHARIDES
MICROORGANISMS
NONMETALS
NORTH AMERICA
NUMERICAL DATA
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLLUTION
POLYSACCHARIDES
POPULATION DYNAMICS
QUANTITY RATIO
RENEWABLE ENERGY SOURCES
RIVERS
SACCHARIDES
SAMPLING
SEDIMENTS
STREAMS
SURFACE WATERS
TURBIDITY
USA
WATER
WATER POLLUTION
WATER QUALITY
WEST VIRGINIA