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Concentration, physical state, and purity of bacterial endotoxin affect its detoxification by ionizing radiation

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3576821· OSTI ID:6776601
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Increasing concentrations of a highly purified bacterial lipopolysaccharide preparation, the U.S. Reference Standard Endotoxin, were exposed to increasing doses of ionizing radiation from a 60Co source. At identical radiation doses both the structural change and Limulus amebocyte lysate (LAL) reactivity were progressively smaller with increasing concentrations of the lipopolysaccharide in an aqueous medium. Under the experimental conditions used, there was a linear relationship between the endotoxin concentration and radiation dose for the structural changes. In contrast to endotoxin in aqueous medium, endotoxin irradiated in its dry state showed no decrease in LAL reactivity and rabbit pyrogenicity. Endotoxin exposed to radiation in water in the presence of albumin showed a much smaller decrease in LAL and pyrogenic activities than expected. The results show that the concentration, physical state, and purity of endotoxin influence its structural and functional alteration by ionizing radiation.
Research Organization:
National Institutes of Health, Bethesda, MD
OSTI ID:
6776601
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 2; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTIGENS
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL RADIATION EFFECTS
CHEMISTRY
COBALT 60
COBALT ISOTOPES
DETOXIFICATION
ENDOTOXINS
ESCHERICHIA COLI
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MATERIALS
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIOISOTOPES
TOXIC MATERIALS
TOXINS
YEARS LIVING RADIOISOTOPES