Small gold-conjugated antibody label: improved resolution for electron microscopy

Journal Article · Fri Apr 24 00:00:00 EDT 1987 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.3563522· OSTI ID:6355329

Hainfeld, J F

A general method has been developed to make the smallest gold-conjugated antibody label yet developed for electron microscopy. It should have wide application in domainal mapping of single molecules or in pinpointing specific molecules, sites, or sequences in supramolecular complexes. It permits electron microscopic visualization of single antigen-binding antibody fragments (Fab') by covalently linking an 11-atom gold cluster to a specific residue on each Fab' such that the antigenic specificity and capacity are preserved. The distance of the gold cluster from the antigen is a maximum of 5.0 nanometers when the undecagold-Fab' probe is used as an immunolabel.

• OSTI ID: 6355329
• Journal Information: Science (Washington, D.C.); (United States), Vol. 236
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
LABELLING
MOLECULAR BIOLOGY
TRACER TECHNIQUES
ANTIGENS
ELECTRON MICROSCOPY
GOLD ISOTOPES
MOLECULES
RESOLUTION
ISOTOPE APPLICATIONS
ISOTOPES
MICROSCOPY
550201* - Biochemistry- Tracer Techniques