Size heterogeneity of epidermal growth factor in human body fluids

Journal Article · Mon Jun 29 00:00:00 EDT 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90069-5· OSTI ID:6354860

Pesonen, K; Viinikka, L; Koskimies, A; Banks, A R; Nicolson, M; Perheentupa, J

The authors measured the concentration of immunoreactive (IR) hEGF in various body fluids by radioimmunoassay (RIA) and evaluated its size heterogeneity by size exclusion high performance liquid chromatography combined with RIA or with time-resolved immunofluorometric assay (TR-IFMA). Mean concentration was 80 ng/ml in urine, 65 ng/ml in milk, 50 ng/ml in seminal plasma, 25 ng/ml in armpit sweat, 1 ng/ml in breast sweat, 0.3 ng/ml in third-trimester amniotic fluid, 3 ng/ml in saliva, 1.5 ng/ml in tears and 0.3 ng/ml in gastric juice. All the fluids except armpit sweat and gastric juice contained two to five molecular sizes of IR-hEGF. As well as the 6200-dalton (6.2kDa) hEGF the authors found at least four other different molecular sizes with approximate weights of greater than or equal to300, 150, 70 and 20 kDa. The authentic 6.2kDa form made up >90% of the total IR-hEGF in all except the amniotic fluid where its proportion was 71%, and the seminal plasma where the proportion could not be determined. 18 references, 1 figure, 1 table.

• Research Organization: Univ. of Helsinki, Finland
• OSTI ID: 6354860
• Journal Information: Life Sci.; (United States), Vol. 40:26
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
BODY FLUIDS
PEPTIDE HORMONES
MOLECULAR WEIGHT
RADIOIMMUNOASSAY
EXPERIMENTAL DATA
IMMUNE REACTIONS
LIQUID COLUMN CHROMATOGRAPHY
MAN
MOLECULAR BIOLOGY
ANIMALS
BIOLOGICAL MATERIALS
CHROMATOGRAPHY
DATA
HORMONES
IMMUNOASSAY
IMMUNOLOGY
INFORMATION
ISOTOPE APPLICATIONS
MAMMALS
MATERIALS
NUMERICAL DATA
PRIMATES
RADIOASSAY
RADIOIMMUNOLOGY
SEPARATION PROCESSES
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics