Regulation of in vitro PWM-induced IgG secretion in humans
Journal Article
·
· Cell. Immunol.; (United States)
PWM-induced differentiation of human PBMC into immunoglobulin (Ig) secreting cells is a widely used model of in vivo IgG secretion. We examined the relationship between the amount of IgG secreted in PBMC cultures obtained from individuals who consistently secrete either very high (HR) or very low amounts (LR) of IgG and various in vitro immune function assays. The PBMC obtained from LR contained a higher ratio of cells expressing the T-suppressor/inducer to T-helper/inducer phenotype. The autologous mixed lymphocyte response was lower and the amount of in vivo radiation sensitive suppression was higher in the LR than in the HR. LR E- cells secreted less IgG than the HR E- cells when both were mixed with heterologous HR E+ cells. Monocyte depletion reduced IgG secretion in both LR and HR. These results suggest that each of the subsets Ts, Th (Thi, Tsi), and B lymphocytes are involved in the regulation of PWM-induced Ig secretion and that the functions of each of these subsets differ in HR and LR individuals.
- Research Organization:
- Univ. of British Columbia, Vancouver (Canada)
- OSTI ID:
- 6351077
- Journal Information:
- Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 118:2; ISSN CLIMB
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL DIFFERENTIATION
CONNECTIVE TISSUE CELLS
GLOBULINS
IMMUNITY
IMMUNOGLOBULINS
IMMUNOSUPPRESSION
IN VITRO
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MITOGENS
MONOCYTES
ORGANIC COMPOUNDS
PHENOTYPE
PRIMATES
PROTEINS
RADIOINDUCTION
SECRETION
SOMATIC CELLS
VERTEBRATES
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL DIFFERENTIATION
CONNECTIVE TISSUE CELLS
GLOBULINS
IMMUNITY
IMMUNOGLOBULINS
IMMUNOSUPPRESSION
IN VITRO
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MITOGENS
MONOCYTES
ORGANIC COMPOUNDS
PHENOTYPE
PRIMATES
PROTEINS
RADIOINDUCTION
SECRETION
SOMATIC CELLS
VERTEBRATES