Continuous ethanol production from Jerusalem artichoke tubers. II. Use of immobilized cells of Kluyveromyces marxianus
Kluyveromyces marxianus UCD (FST) 55-82 cells were immobilized in Na alginate beads and used in a packed-bed bioreactor system for the continuous production of ethanol from the extract of Jerusalem artichoke tubers. Volumetric ethanol productivities of 104 and 80 g ethanol/L/h were obtained at 80 and 92% sugar utilization, respectively. The maximum volumetric ethanol productivity of the immobilized cell bioreactor system was found to be 15 times higher than that of an ordinary continuous-stirred-tank (CST) bioreactor using free cells of Kluyveromyces marxianus. The immobilized cell bioreactor system was operated continuously at a constant dilution rate of 0.66/h for 12 days resulting in only an 8% loss of the original immobilized cell activity, which corresponds to an estimated half-life of ca. 72 days. The maximum specific ethanol productivity and maximum specific sugar uptake rate of the immobilized cells were found to be 0.55 g ethanol/g biomass/h and 1.21 g sugars/g biomass/h, respectively. (Refs. 27).
- Research Organization:
- Dept of Chemical and Biochemical Engineering, Faculty of Engineering Science, The Univ of Western Ontario, London, Ontario, Canada N6A 5B9
- OSTI ID:
- 6347672
- Journal Information:
- Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 24:7; ISSN BIBIA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
BIOCONVERSION
BIOREACTORS
CONTINUOUS CULTURE
ETHANOL
FERMENTATION
FUNGI
HYDROXY COMPOUNDS
IMMOBILIZED CELLS
MICROORGANISMS
ORGANIC COMPOUNDS
PACKED BED
PLANTS
PRODUCTION
TUBERS
YEASTS