Ethanol inhibition kinetics of Kluyveromyces marxianus grown on Jerusalem artichoke juice
The kinetics of ethanol inhibition on cell growth and ethanol production by Kluyveromyces marxianus UCD (FST) 55-82 were studied during batch growth. The liquid medium contained 10% (weight/volume) inulin-type sugars derived from an extract of Jerusalem artichoke (Helianthus tuberosus) tubers, supplemented with small amounts of Tween 80, oleic acid, and corn steep liquor. Initial ethanol concentrations ranging from 0 to 80 g/liter in the liquid medium were used to study the inhibitory effect of ethanol on the following parameters: maximum specific growth rate (mu max), cell and ethanol yields, and sugar utilization. It was found that as the initial ethanol concentration increased from 0 to 80 g/liter, and maximum specific growth rate of K. marxianus cells decreased from 0.42 to 0.09/hour, whereas the ethanol and cell yields and sugar utilization remained almost constant. A simple kinetic model was used to correlate the mu max results and the rates of cell and ethanol production, and the appropriate constants were evaluated. (Refs. 22).
- Research Organization:
- Chemical and Biochemical Engineering, Faculty of Engineering Science, The Univ of Western Ontario, London, Ontario N6A 5B9, Canada
- OSTI ID:
- 6247405
- Journal Information:
- Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 44:6; ISSN AEMID
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
ALCOHOLS
BIOCONVERSION
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBOHYDRATES
ETHANOL
FERMENTATION
FUNGI
HYDROXY COMPOUNDS
INHIBITION
KINETICS
MICROORGANISMS
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SYNTHESIS
TUBERS
YEASTS