Hydrogenase activity in the thermophile mastigocladus laminosus

Journal Article · Wed Jun 30 00:00:00 EDT 1982 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(82)91239-6· OSTI ID:6342555

Benemann, J R; Miyamoto, K; Hallenbeck, P C; Murry, M A

Hydrogenase activity in the thermophilic cyanobacterium, Mastigocladus laminosus was studied both in vivo and in vitro. In vivo hydrogen consumption required oxygen but not light, was about ten-fold higher than in mesophilic cyanobacteria, and was relatively insensitive to carbon monoxide. H/sub 2/-supported acetylene reduction in reductant-limited cultures was a light-dependent, but O/sub 2/-independent reaction. In vitro hydrogen evolution was unaffected by carbon monoxide, and this activity could be partially purified using a procedure developed for Anabaena cylindrica.

• Research Organization: Sanitary Engineering Research Lab., Berkeley, CA
• OSTI ID: 6342555
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 106:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALGAE
ENZYME ACTIVITY
CARBON MONOXIDE
HYDROGEN
HYDROGENASES
IN VITRO
IN VIVO
NITROGEN FIXATION
OXYGEN
THERMOPHILIC CONDITIONS
VISIBLE RADIATION
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
ELECTROMAGNETIC RADIATION
ELEMENTS
ENZYMES
NONMETALS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PLANTS
RADIATIONS
550200* - Biochemistry
550700 - Microbiology