Parallel decrease of erythrocyte membrane deformability and spectrin solubility at low pH

Journal Article · Mon Jan 01 04:00:00 EST 1979 · Blood; (United States)

OSTI ID:6341236

Smith, B D; La Celle, P L

Reduction of pH over the range 6.0--4.5 results in a decrease of erythrocyte deformability in parallel with the induced progressive sphericity of cells. At low deformation rates employed, increase of hemoglobin viscosity was not significant. A decrease of membrane elasticity was detected in cells when sphering, the major determinant of cellular deformability, was prevented at pH 5.0 by hyperosmotic medium. The pronounced change of deformability and the reduced elasticity occurred at calculated intracellular pH values at which solubility in vitro of extracted erythrocyte spectrin is also markedly reduced. The parallel decrease of deformability and spectrin solubility supports the hypothesis that spectrin aggregation may contribute to regulation of erythrocyte deformability through effects on shape and membrane elastic characteristics.

• Research Organization: Univ. of Rochester School of Medicine and Dentistry, NY
• OSTI ID: 6341236
• Journal Information: Blood; (United States), Journal Name: Blood; (United States) Vol. 53:1; ISSN BLOOA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
DATA
DATA FORMS
ELASTICITY
ERYTHROCYTES
EXPERIMENTAL DATA
GLOBIN
GRAPHS
HEMOGLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INFORMATION
MECHANICAL PROPERTIES
MEMBRANES
MORPHOLOGICAL CHANGES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PH VALUE
PIGMENTS
PORPHYRINS
PROTEINS
SOLUBILITY
TENSILE PROPERTIES
VISCOSITY