Identification by peptide analysis of the spectrin-binding protein in human erythrocytes
Journal Article
·
· J. Biol. Chem.; (United States)
OSTI ID:7008159
One-dimensional and two-dimensional peptide-mapping techniques are used to identify the protein which gives rise to the 72,000 dalton ..cap alpha..-chymotryptic fragment previously shown to be the membrane attachment site for spectrin. Limited proteolysis of erythrocyte membranes is shown to generate Band 3', another polypeptide which has been associated with spectrin-binding activity. It is concluded that Band 2.1 and possibly some or all of the other, related polypeptides which electrophorese in the 2 region is (are) the spectrin-binding protein(s) of the human erythrocyte.
- Research Organization:
- Harvard Univ., Cambridge, MA
- DOE Contract Number:
- AC02-76EV02423
- OSTI ID:
- 7008159
- Journal Information:
- J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 254:7; ISSN JBCHA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Spectrin promotes the association of F-actin with the cytoplasmic surface of the human erythrocyte membrane
Spectrin: current understanding of its physical, biochemical, and functional properties
Increased (/sup 32/P)-phosphorylation of tryptic peptides of erythrocyte spectrin in Duchenne muscular dystrophy
Journal Article
·
Sat Jan 31 23:00:00 EST 1981
· J. Cell Biol.; (United States)
·
OSTI ID:5247562
Spectrin: current understanding of its physical, biochemical, and functional properties
Journal Article
·
Wed Dec 31 23:00:00 EST 1975
· Life Sci.; (United States)
·
OSTI ID:7304996
Increased (/sup 32/P)-phosphorylation of tryptic peptides of erythrocyte spectrin in Duchenne muscular dystrophy
Journal Article
·
· Muscle Nerve; (United States)
·
OSTI ID:5245930
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ERYTHROCYTES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
MAMMALS
MAN
MATERIALS
MEMBRANES
MOLECULAR STRUCTURE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
PROTEOLYSIS
RADIOISOTOPES
RECEPTORS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
ERYTHROCYTES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
MAMMALS
MAN
MATERIALS
MEMBRANES
MOLECULAR STRUCTURE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
PROTEOLYSIS
RADIOISOTOPES
RECEPTORS
VERTEBRATES