Stability of the phenotypic reversion of x-ray transformed C3H/10T1/2 cells depends on cellular proliferation after subcultivation at low cell density

Journal Article · Thu Dec 31 23:00:00 EST 1981 · Carcinogenesis (N.Y.); (United States)

DOI:https://doi.org/10.1093/carcin/3.9.1057· OSTI ID:6340171

Brouty-Boye, D; Gresser, I; Bandu, M T ^[1]

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1. Centre National de la Recherche Scientifique, 94 - Villejuif (France). Inst. de Recherches Scientifiques sur le Cancer

Reversion from the transformed to the non-transformed phenotype could be obtained by seeding X-ray transformed C3H/10T1/2 cells at low cell density. Cloned revertant cells of varying degrees of reversion were obtained depending on the time they were isolated after one subculture at low cell density. Most of the revertants isolated 7 and 10 days after seeding at very low cell density eventually returned to the transformed phenotype when passaged serially at high cell density. In contrast, 25-35% of the revertants isolated 17-20 days after seeding at low cell density maintained the non-transformed phenotype despite subsequent serial passages at high cell density. The finding that there was a direct relationship between the time during which transformed cells seeded at low cell density multiplied and the number of stable revertant clones obtained, suggests the possibility that reversion from the transformed to the non-transformed phenotype may be a multistep process. Revertant cells displayed a chromosomal pattern characteristic of the transformed cells rather than that of the parental non-transformed 10T1/2 cells.

• OSTI ID: 6340171
• Journal Information: Carcinogenesis (N.Y.); (United States), Journal Name: Carcinogenesis (N.Y.); (United States) Vol. 3:9; ISSN CRNGD
• Country of Publication: United States
• Language: English

Related Subjects

560121* -- Radiation Effects on Cells-- External Source-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
FIBROBLASTS
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IONIZING RADIATIONS
MAMMALS
MICE
MUTANTS
ONCOGENIC TRANSFORMATIONS
PHENOTYPE
RADIATION EFFECTS
RADIATIONS
REVERTANTS
RODENTS
SOMATIC CELLS
STABILITY
VERTEBRATES
X RADIATION