Cell density dependence of focus formation in the C3H/10T1/2 transformation assay
Some of the dynamics of neoplastic transformation in vitro have been studied with the use of benzo(a)pyrene as the carcinogen in the C3H/10T1/2 morphological transformation assay. Experiments that involved the dispersion of cells into new culture dishes at various times after carcinogen treatment have shown that no change in the fraction of potentially transformed cells occurs while cultures grow to form a confluent monolayer, that little or no change in the fraction of potentially transformed cells occurs for approximately 3 weeks after confluence is attained, and that this fraction increases rapidly some 7 weeks after BP treatment. When confluent benzo(a)pyrene-treated cultures are dispersed in new culture dishes prior to the onset of growth toward focus formation, the formation of transformed foci is suppressed at high cell densities of seeding. This phenomenon is independent of the total number of divisions undergone by cells after treatment. We suggest that phenotypic expression of morphological transformation is dependent on colony interactions in the C3H/10T1/2 system, which we do not yet understand, but which are independent of time posttreatment expressed either in cell generations or absolute time.
- Research Organization:
- Massachusetts Inst. of Tech., Cambridge
- OSTI ID:
- 6253922
- Journal Information:
- Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 37; ISSN CNREA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Genome lability in radiation-induced transformants of C3H 10T1/2 mouse fibroblasts
Immunologic and biochemical detection of cell surface alterations following morphological transformation or mutation of C3H/10T1/2 C1 8 cells by chemical carcinogens
Related Subjects
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
BIOASSAY
BIOLOGICAL EFFECTS
CARCINOGENS
CELL CULTURES
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
DISEASES
FIBROBLASTS
HYDROCARBONS
IN VITRO
MAMMALS
MICE
NEOPLASMS
ORGANIC COMPOUNDS
PHENOTYPE
RODENTS
SOMATIC CELLS
VERTEBRATES