Chromosomal organization of the human V[sub H]4 gene family: Location of individual gene segments
- Virginia Mason Research Center, Seattle, WA (United States)
- Virginia Mason Research Center, Seattle, WA (United States) Univ. of Washington, Seattle (United States)
To investigate the organization and evolution of V[sub H] gene segments, the authors characterized the elements belonging to the V[sub H]4 gene family from the germline of a single subject. One hundred sixty V[sub H]4-carrying [lambda]-phage clones were isolated from a genomic library. A combination of hybridization and sequence analysis yielded 13 distinct V[sub H]4 clones. Six of these elements had one or more nucleotide substitutions that distinguished them from previously identified V[sub H]4 genes, whereas seven elements were identical to previously described V[sub H]4 genes. In four of the six new sequences, nucleotide substitutions resulted in amino acid replacements. One pseudogene was identified. On the basis of sequence-specific hybridization using oligonucleotide probes corresponding to these sequences, each of the elements could be assigned to a specific band in a Bg/II digest. Since the V[sub H]4-carrying Bg/II bands have been mapped in genomic DNA, it was also possible to assign chromosomal locations to the specific V[sub H]4 elements. The results indicate that the majority of V[sub H]4 elements are located in a region of approximately 500 kb, extending from approximately 500 to 1,000 kb 5[prime] of the J[sub H] locus on chromosome 14. The distribution of shared structural motifs among the V[sub H]4 elements indicates that the V[sub H]4 gene family has evolved through repeated duplication and gene conversion events. 40 refs., 6 figs., 2 tabs.
- OSTI ID:
- 6337001
- Journal Information:
- Journal of Immunology; (United States), Vol. 150:7; ISSN 0022-1767
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GENES
BIOLOGICAL EVOLUTION
SPATIAL DISTRIBUTION
HUMAN CHROMOSOME 14
MAPPING
IMMUNOGLOBULINS
DNA HYBRIDIZATION
DNA SEQUENCING
GENETIC VARIABILITY
HUMAN CHROMOSOMES
BIOLOGICAL VARIABILITY
CHROMOSOMES
DISTRIBUTION
GLOBULINS
HYBRIDIZATION
ORGANIC COMPOUNDS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
550400* - Genetics