Nucleotide sequence analysis of 77. 7 kb of the human V[sub [beta]] T-cell receptor gene locus: Direct primer-walking using cosmid template DNAs
- Upjohn Co., Kalamazoo, MI (United States)
- Univ. of Victoria, British Columbia (Canada)
- Univ. of Washington, Seattle, WA (United States)
The nucleotide sequence of 77.7 kb from the human T-cell receptor [beta]-chain locus was determined directly from three overlapping cosmid clones using the primer-walking approach. Computer-aided analyses of this sequence reveal the presence of at least 11 genic regions that are closely related to the human T-cell receptor [beta] variable region (TCRBV) gene family. These include five germline sequences that have previously been determined, V[sub [beta]]21.2, V[sub [beta]]8.1, V[sub [beta]]8.2, V[sub [beta]]8.3, and V[sub [beta]]16, and four whose sequences have partially been determined at the mRNA level, V[sub [beta]]6, V[sub [beta]]23, V[sub [beta]]12.2, V[sub [beta]]24. The two remaining V[sub [beta]] Ter-related sequences have eluded discovery by cDNA and RT-PCR cloning and genomic blot hybridization methods. These two V[sub [beta]] Tcr-related genes lack >75% nucleotide sequence identity with any other V[sub [beta]] TCR gene member and therefore, by convention, are referred to as new subfamily members V[sub [beta]]25 and V[sub [beta]]26. This lack of shared identity with other subfamily members explains why they were not detected by hybridization. The promoter regions of these V[sub [beta]] Tcr genes contain the conserved Tcr decamer element located between 80 and 110 bp 5[prime] of the translation start site, generally near a putative TATAA promoter element. Sequence analysis also reveals that a 3.3-kb duplication unit was involved in the recombination event that produced the closely related V[sub [beta]]8.1 and 8.2 gene subfamily members. This sequenced region of the V[sub [beta]] locus contains an average number of repetitive DNA elements (21 Alu, three L1, three MER, and three retro-virus-related elements). 87 refs., 3 figs., 6 tabs.
- DOE Contract Number:
- FG03-91ER61182
- OSTI ID:
- 6789519
- Journal Information:
- Genomics; (United States), Vol. 20:2; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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