Biodegradation of 2,4,5-trichlorophenoxyacetic acid in liquid culture and in soil by the white rot fungus phanerochaete chrysosporium
Extensive biodegradation of (14)C-2,4,5-trichlorophenoxyacetic acid ((14)C-2,4,5-T) by the white rot fungus Phanerochaete chrysosporium was demonstrated in nutrient nitrogen-limited aqueous cultures and in (14)C-2,4,5-T-contaminated soil inoculated with this fungus and supplemented with ground corn cobs. After incubation of (14)C-2,4,5-T with aqueous cultures of the fungus for 30 days, 62.0% - or + 2.0% of the (14)C-2,4,5-T initially present was degraded to (14)CO2. Mass balance analysis demonstrated that water soluble metabolites were formed during degradation, and HPLC and thin layer chromatography (TLC) of methylene chloride-extractable material revealed the presence of polar and non-polar (14)C-2,4,5-T initially present in cultures remained as undegraded (14)C-2,4,5-T. In incubations composed of (14)C-2,4,5-T-contaminated soil, ground corn cobs, and 40% (w/w) water, 32.5%-3.6% of the (14)C-2,4,5-T initially present was converted to (14)CO2 after 30 days of incubation. These results suggest that it may be possible to develop practical systems based on the use of the fungus to detoxify 2,4,5-T-contaminated water and soil.
- Research Organization:
- Utah State Univ., Logan, UT (United States)
- OSTI ID:
- 6336037
- Report Number(s):
- PB-93-191542/XAB; CNN: EPA-R-813369; EPA-R-814448
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
540220* -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
550501 -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGRICULTURAL WASTES
BIODEGRADATION
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON OXIDES
CHALCOGENIDES
CHEMICAL EFFLUENTS
CHEMICAL REACTIONS
CHEMICAL WASTES
CHEMISTRY
CHROMATOGRAPHY
CONTAMINATION
CULTURE MEDIA
DECOMPOSITION
ELEMENTS
EUMYCOTA
FUNGI
HERBICIDES
INCUBATION
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
MASS BALANCE
METABOLITES
NITROGEN
NONMETALS
NONRADIOACTIVE WASTES
ORGANIC WASTES
OXIDES
OXYGEN COMPOUNDS
PESTICIDES
PHANEROCHAETE
PLANTS
POLLUTANTS
SEPARATION PROCESSES
SOIL CHEMISTRY
THIN-LAYER CHROMATOGRAPHY
WASTES