Biodegradation of polycyclic aromatic hydrocarbons by phanerochaete chrysosporium

Technical Report · Sun Jan 01 04:00:00 EST 1989

OSTI ID:6331836

Bumpus, J A

The ability of the white rot fungus Phanerochaete chrysosporium to degrade polycyclic aromatic hydrocarbons (PAHs) that are present in anthracene oil (a distillation product obtained from coal tar) was demonstrated. Analysis by capillary gas chromatography and high-performance liquid chromatography showed that at least 22 PAHs, including all of the most abundant PAH components present in anthracene oil, underwent 70 to 100% disappearance during 27 days of incubation with nutrient nitrogen-limited cultures of this fungus. Because phenanthrene is the most abundant PAH present in anthracene oil, this PAH was selected for further study. In experiments in which ((14)C) phenanthrene was incubated with cultures of P. chrysosporium containing anthracene oil for 27 days, it was shown that 7.7% of the recovered radiolabeled carbon originally present in ((14)C) phenanthrene was metabolized to (14)CO2 and 25.2% was recovered from the aqueous fraction, while 56.1 and 11.0% were recovered from the methylene chloride and particulate fractions, respectively.

• Research Organization: Utah State Univ., Logan, UT (United States)
• OSTI ID: 6331836
• Report Number(s): PB-93-191591/XAB
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540220 -- Environment
Terrestrial-- Chemicals Monitoring & Transport-- (1990-)
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANTHRACENE
AROMATICS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIODEGRADATION
CARBON 14
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON ISOTOPES
CARBON OXIDES
CHALCOGENIDES
CHEMICAL REACTIONS
CHLORIDES
CHLORINE COMPOUNDS
CHROMATOGRAPHY
COAL TAR
CONDENSED AROMATICS
DECOMPOSITION
DEVELOPED COUNTRIES
DISTILLATION
ELEMENTS
EUMYCOTA
EVEN-EVEN NUCLEI
FUNGI
GAS CHROMATOGRAPHY
HALIDES
HALOGEN COMPOUNDS
HIGH PRESSURE
HYDROCARBONS
INCUBATION
ISOTOPES
LIGHT NUCLEI
METHYLENE CHLORIDE
NITROGEN
NONMETALS
NORTH AMERICA
NUCLEI
OILS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OTHER ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PHANEROCHAETE
PHENANTHRENE
PLANTS
POLYCYCLIC AROMATIC HYDROCARBONS
RADIOISOTOPES
SEPARATION PROCESSES
TAR
USA
YEARS LIVING RADIOISOTOPES