Fractionation of radioactivity in the milk of goats administered UC-aflatoxin B1

Journal Article · Wed May 01 00:00:00 EDT 1985 · J. Assoc. Off. Anal. Chem.; (United States)

OSTI ID:6335523

Goto, T; Hsieh, D P

A detailed fractionation of radioactivity in the milk of goats administered UC-aflatoxin B1 at low doses was performed. The milk collected in the first 24 h following dosing contained radioactivity equivalent to 0.45-1.1% of the dose given. The radioactivity in each sample was partitioned into 4 fractions: ether, protein, dichloromethane, and water-alcohol. Over 80% of the radioactivity was detected in the dichloromethane fraction, of which over 95% was attributable to aflatoxin M1. No aflatoxin B1 or other known aflatoxin metabolites were detected in any fraction. The results indicate that the major metabolite of aflatoxin B1 in goat milk is aflatoxin M1 and that other metabolites, including conjugates, are of minor significance.

• Research Organization: Univ. of California, Davis
• OSTI ID: 6335523
• Journal Information: J. Assoc. Off. Anal. Chem.; (United States), Vol. 3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AFLATOXIN
BIOCHEMISTRY
METABOLITES
CARBON 14 COMPOUNDS
TRACER TECHNIQUES
GOATS
MILK
ANIMALS
ANTIGENS
BIOLOGICAL MATERIALS
BODY FLUIDS
CHEMISTRY
DOMESTIC ANIMALS
FOOD
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
RUMINANTS
TOXIC MATERIALS
TOXINS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques