Identification of 1,25-dihydroxyvitamin D3 receptors and activities in muscle

Journal Article · Thu Jul 25 04:00:00 EDT 1985 · J. Biol. Chem.; (United States)

OSTI ID:6334472

Simpson, R U; Thomas, G A; Arnold, A J

Cytosols from cultured myoblast cells prepared in high salt possesses receptor like proteins for 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) that sediment in the 3.2 S region of sucrose gradients. These receptors were characterized as having high affinity for 1,25-(OH)2D3 and are in low capacity. Analog competition for receptor binding revealed that 1,25-(OH)2D3 was more potent than 24,25-(OH)2D3, or 25-(OH)2D3 for displacement of 1,25-(OH)2(TH)D3 from these 3.2 S region sedimenting receptors. Furthermore, the receptor proteins had affinity for DNA and eluted from Sephacryl S-200 as a macromolecule with Stokes radius (Rs) of 32 A. High salt cytosol from collagenase-dispersed skeletal muscle cells was also found to possess a 3.2 S 1,25-(OH)2D3 receptor-like protein. The 1,25-(OH)2D3 receptor concentration was found to down-regulate by 50-70% when cells were stimulated to differentiate to myotubes by lowering fetal calf serum to 5% of the medium. Moreover, the authors demonstrated that 1,25-(OH)2D3 can inhibit DNA synthesis and cell proliferation of the G-8 myoblast cells in a dose-dependent manner. The data support the possibility that muscle is a target tissue for 1,25-(OH)2D3 and the hormone may act to initiate terminal differentiation of myoblast cells.

• Research Organization: Univ. of Michigan Medical School, Ann Arbor
• OSTI ID: 6334472
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 15; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ALKALINE EARTH METALS
ANIMAL CELLS
ANIMALS
CALCIUM
CELL DIFFERENTIATION
CELL DIVISION
DNA
ELEMENTS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
METALS
MICE
MUSCLES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIORECEPTOR ASSAY
RATS
RECEPTORS
RODENTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES
VITAMIN D
VITAMINS