Evaluation of a photolabile derivative of 1,25-dihydroxyvitamin D3 as a photoaffinity probe for 1,25-dihydroxyvitamin-D3 receptor in chick intestinal cytosol
Journal Article
·
· Biochem. Biophys. Res. Commun.; (United States)
The authors evaluated the viability of 1 alpha, 25-dihydroxyvitamin D3-3 beta-(N-(4-azido-2-nitrophenyl)glycinate) (1,25-(OH)2-D3-ANG), an analog of 1 alpha, 25-dihydroxyvitamin D3 (1,25-(OH)2-D3) as a photoaffinity probe for 1,25-(OH)2-D3 receptor in chick intestinal cytosol. A competitive-binding assay revealed that chick intestinal cytosolic 1,25-(OH)2- D3 receptor bound to 1,25-(OH)2-D3-ANG approximately 20-times less effectively than it did to 1,25-(OH)2-D3. Irradiation of 1,25-(OH)2-D3- ANG in the presence of chick intestinal cytosolic preparation significantly diminished subsequent binding to /sup 3/H-1,25-(OH)2-D3, suggesting that the photoaffinity analog was covalently attached to the receptor. Therefore the nitroarylazide derivative of 1,25-(OH)2-D3 may be a valuable photoaffinity probe for the characterization of the 1,25-(OH)2-D3 receptor.
- Research Organization:
- Tufts Univ., Boston, MA
- OSTI ID:
- 6136345
- Journal Information:
- Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 1; ISSN BBRCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AZIDES
BIRDS
BODY
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
CHICKENS
DECOMPOSITION
DIGESTIVE SYSTEM
ELECTROMAGNETIC RADIATION
FOWL
GASTROINTESTINAL TRACT
INTESTINES
LABELLED COMPOUNDS
MEMBRANE PROTEINS
NITROGEN COMPOUNDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RADIOLYSIS
RECEPTORS
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
VERTEBRATES
VITAMIN D
VITAMINS
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AZIDES
BIRDS
BODY
CHEMICAL RADIATION EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
CHICKENS
DECOMPOSITION
DIGESTIVE SYSTEM
ELECTROMAGNETIC RADIATION
FOWL
GASTROINTESTINAL TRACT
INTESTINES
LABELLED COMPOUNDS
MEMBRANE PROTEINS
NITROGEN COMPOUNDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION CHEMISTRY
RADIATION EFFECTS
RADIATIONS
RADIOLYSIS
RECEPTORS
TRITIUM COMPOUNDS
ULTRAVIOLET RADIATION
VERTEBRATES
VITAMIN D
VITAMINS