Selection of a Pseudomonas cepacia strain constitutive for the degradation of trichloroethylene

Journal Article · Tue Dec 01 04:00:00 EST 1992 · Applied and Environmental Microbiology; (United States)

OSTI ID:6332066

Shields, M S ^[1]; Reagin, M J ^[2]

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1. Univ. of West Florida, Pensacola (United States)
2. Technical Resources Inc., Gulf Breeze, FL (United States) Environmental Protection Agency, Gulf Breeze, FL (United States)

Groundwater contamination by organic pollutants, particularly volatile organics including TCE, DCE, I,I-DCE, and vinyl chloride, is of concern throughout the industrialized world. The capability of biologically degrading such contaminants at the site of pollution should be of considerable treatment value. However, all TCE-degrading bacterial, with one exception, require the addition of an exogenous inducer substrate such as toluene, phenol, methane, isoprene, propane or 2,4-dichlorophenoxyacetic acid to induce the enzymes require for the degradation TCE. This paper describes a Tn5-induced mutant of Pseudomonas cepacia G4 (Tom-) that does not express toluene ortho-monooxygenase (TOM) but spontaneously reverts to the constitutive expression of TOM. This revertant no longer requires aromatic induction of the TOM pathway enzyme(s) in order to degrade TCE.

• OSTI ID: 6332066
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 58:12; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

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