Constitutive degradation of trichloroethylene by an altered bacterium in a gas-phase bioreactor. Book chapter
Pseudomonas cepacia G4 expresses a unique toluene orth-monooxygenase (Tom) that enables it to degrade toluene and trichloroethylene (TCE). Transposon mutants of G4 have been isolated that constitutively express Tom. Two fixed-film bioreactor designs were investigated for the exploitation of one such constitutive strain (G4 PR1) in the degradation of vapor-phase TCE. DNA probe analysis indicates that the genes responsible for the toluene catabolic pathway are located on a large plasmid of G4 (> or = 150 kb) termed pG4L. The authors propose that pG4L will serve as a archetype for a new class of catabolic plasmid known as Tom, which encodes an ortho-hydroxylation pathway for the degradation of benzene, toluene, o-xylene, cresols, and phenol.
- Research Organization:
- University of West Florida, Pensacola, FL (United States)
- OSTI ID:
- 6769064
- Report Number(s):
- PB-95-122941/XAB; CNN: EPA-68-03-3479; USAF-F-08635-92-C-0103
- Country of Publication:
- United States
- Language:
- English
Similar Records
Mutants of Pseudomonas cepacia G4 defective in catabolism of aromatic compounds and trichloroethylene
Studies in applying Pseudomonas cepacia PR1-(pTOM{sub 31C}) to a TCE bioreactor
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54 ENVIRONMENTAL SCIENCES
540320 -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
BIODEGRADATION
CHEMICAL REACTIONS
CHLORINATED ALIPHATIC HYDROCARBONS
CONTROL
DECOMPOSITION
HALOGENATED ALIPHATIC HYDROCARBONS
HYDROGEN COMPOUNDS
LIQUID WASTES
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
OXYGEN COMPOUNDS
POLLUTION CONTROL
WASTE WATER
WASTES
WATER
WATER POLLUTION CONTROL