Respiratory failure following anti-lung serum: study on mechanisms associated with surfactant system damage

Journal Article · Thu Jan 01 04:00:00 EST 1987 · Exp. Lung Res.; (United States)

DOI:https://doi.org/10.3109/01902148709062839· OSTI ID:6328773

Lachmann, B; Hallman, M; Bergmann, K C

Within 2 minutes intravenous anti-lung serum (ALS) into guinea pig induces a respiratory failure that is fatal within 30 min. The relationship between surfactant, alveolar-capillary permeability and respiratory failure was studied. Within two minutes ALS induced a leak in the alveolar-capillary barrier. Within 30 minutes 28.3% (controls, given normal rabbit serum: 0.7%) of iv /sup 131/I-albumin, and 0.5% (controls 0.02%) of iv surfactant phospholipid tracer were recovered in bronchoalveolar lavage. Furthermore, 57% (controls 32%) of the endotracheally administered surfactant phospholipid became associated with lung tissue and only less than 0.5% left the lung. The distribution of proteins and phospholipids between the in vivo small volume bronchoalveolar lavages and the ex vivo bronchoalveolar lavages were dissimilar: 84% (controls 20%) of intravenously injected, lavageable /sup 131/I-albumin and 23% (controls 18%) of total lavageable phospholipid were recovered in the in vivo small volume bronchoalveolar lavages. ALS also decreased lavageable surfactant phospholipid by 41%. After ALS the minimum surface tension increased. The supernatant of the lavage increased the minimum surface tension of normal surfactant. In addition, the sediment fraction of the lavage had slow surface adsorption, and a marked reduction in 35,000 and 10,000 MW peptides. Exogenous surfactant ameliorated the ALS-induced respiratory failure. We propose that inhibition, altered intrapulmonary distribution, and dissociation of protein and phospholipid components of surfactant are important in early pathogenesis of acute respiratory failure.

• Research Organization: Univ. of Helsinki, Finland
• OSTI ID: 6328773
• Journal Information: Exp. Lung Res.; (United States), Journal Name: Exp. Lung Res.; (United States) Vol. 2; ISSN EXLRD
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CAPILLARIES
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DISEASES
ESTERS
GUINEA PIGS
INTERMEDIATE MASS NUCLEI
IODINE 131
IODINE ISOTOPES
IRRIGATION
ISOTOPES
LIPIDS
MAMMALS
MOLECULAR WEIGHT
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PATHOGENESIS
PERMEABILITY
PHOSPHOLIPIDS
PROTEINS
RADIOISOTOPES
RESPIRATORY SYSTEM DISEASES
RODENTS
SURFACTANTS
TOXICITY
VERTEBRATES