DNA polymerase III of Escherichia coli is required for UV and ethyl methanesulfonate mutagenesis

Journal Article · Mon Jun 01 04:00:00 EDT 1987 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.84.12.4195· OSTI ID:6327883

Hagensee, M E; Timme, T L; Bryan, S K; Moses, R E

Strains of Escherichia coli possessing the pcbA1 mutation, a functional DNA polymerase I, and a temperature-sensitive mutation in DNA polymerase III can survive at the restrictive temperature (43 degrees C) for DNA polymerase III. The mutation rate of the bacterial genome of such strains after exposure to either UV light or ethyl methanesulfonate was measured by its rifampicin resistance or amino acid requirements. In addition, Weigle mutagenesis of preirradiated lambda phage was also measured. In all cases, no increase in mutagenesis was noted at the restrictive temperature for DNA polymerase III. Introduction of a cloned DNA polymerase III gene returned the mutation rate of the bacterial genome as well as the Weigle mutagenesis to normal at 43 degrees C. Using a recA-lacZ fusion, the SOS response after UV irradiation was measured and found to be normal at the restrictive and permissive temperature for DNA polymerase III, as was induction of lambda prophage. Recombination was also normal at either temperature. Our studies demonstrate that a functional DNA polymerase III is strictly required for mutagenesis at a step other than SOS induction.

• Research Organization: Baylor College of Medicine, Houston, TX
• OSTI ID: 6327883
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 12; ISSN PNASA
• Country of Publication: United States
• Language: English

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560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DNA POLYMERASES
DOSE-RESPONSE RELATIONSHIPS
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ESCHERICHIA COLI
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GENE RECOMBINATION
GENES
GENETIC EFFECTS
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MUTAGENESIS
MUTAGENS
MUTATIONS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
RADIATION EFFECTS
RADIATIONS
REACTION KINETICS
SULFONIC ACID ESTERS
TEMPERATURE DEPENDENCE
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ULTRAVIOLET RADIATION