Kinetics of intraventricularly injected trace amines and their deuterated isotopomers

Journal Article · Sat Oct 01 00:00:00 EDT 1988 · Neurochem. Res.; (United States)

DOI:https://doi.org/10.1007/BF00970766· OSTI ID:6326515

Durden, D A; Nguyen, T V; Boulton, A A

Intraventricular injection into the rat brain of four trace amines and a catecholamine resulted in rapid exponential loss of the amines in the first 30 minutes after injection. The half-lives were: phenylethylamine 3.8 min, para-tyramine 5.1 min, meta-tyramine 7.4 min and dopamine 8.0 min. Tryptamine showed a biphasic loss with half-lives of 4.7 min (over the 5 to 10 min period) and 14.1 min (10 to 30 min). The half-lives were substantially increased by deuterium labeling at the alpha carbon position: phenylethylamine 4.8 min, para-tyramine 8.8 min, meta-tyramine 14.1 min, dopamine 13.0 min and tryptamine 6.0 min (5 to 10 min period) and 28.7 min (10 to 20 min). The loss of the amines was reduced by monoamine oxidase inhibition by pargyline hydrochloride and the deuterium isotope effect was abolished. It is noteworthy that the half-life of dopamine was similar to those of the trace amines in this time period and that the trace amine half-lives after i.v. injection was longer than those obtained from measurements of increases of concentrations of endogenous amines after MAOI in vivo and that of dopamine shorter than values calculated from turnover measurements.

• Research Organization: Univ. of Saskatchewan, Saskatoon (Canada)
• OSTI ID: 6326515
• Journal Information: Neurochem. Res.; (United States), Vol. 13:10
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMINES
BIOLOGICAL HALF-LIFE
DEUTERIUM
ISOTOPE EFFECTS
BIOCHEMICAL REACTION KINETICS
BRAIN
CATECHOLAMINES
DOPAMINE
RATS
TRACE AMOUNTS
TRACER TECHNIQUES
TRYPTAMINES
TYRAMINE
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
HYDROXY COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
PYRROLES
REACTION KINETICS
RODENTS
STABLE ISOTOPES
SYMPATHOMIMETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques