Structural alteration in the MYB protooncogene and deletion within the gene encoding. cap alpha. -type protein kinase C in human melanoma cell lines

Journal Article · Fri Jan 01 00:00:00 EST 1988 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.85.1.74· OSTI ID:6326189

Linnebach, A J; Huebner, K; Reddy, E K; Herlyn, M; Parmiter, A H; Nowell, P C; Koprowski, H

A correlative study was done to determine possible relationships between nonrandom aberrations in chromosomes 1, 6, and 7 occurring in human cutaneous malignant melanoma and the structure of oncogenes as well as specific genes encoding growth factors and growth factor receptors. Thirty cell lines derived from primary or metastatic melanomas of 28 patients were analyzed by Southern blotting with nick-translated probes for 28 different genes, some of which map near frequent chromosomal breakpoints observed in melanoma. An alteration in the MYB protooncogene was observed in a cell line derived from a primary melanoma in the vertical growth phase, which correlated with a 6q22 chromosomal abnormality. Another primary melanoma cell line had a cytogenetically undetected tumor-specific deletion within the gene for ..cap alpha..-type protein kinase C. Polymorphic alleles for the genes encoding the epidermal growth factor receptor and ..cap alpha..-type protein kinase C were also observed.

• Research Organization: Wistar Institute, Philadelphia, PA (USA)
• OSTI ID: 6326189
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 85:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMAL ABERRATIONS
DNA SEQUENCING
ONCOGENES
MOLECULAR STRUCTURE
RECOMBINANT DNA
HYBRIDIZATION
ELECTROPHORESIS
GENES
GROWTH FACTORS
HETEROCHROMOSOMES
MELANOMAS
PATIENTS
PHOSPHORUS 32
PHOSPHOTRANSFERASES
RECEPTORS
TUMOR CELLS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHROMOSOMES
DAYS LIVING RADIOISOTOPES
DISEASES
DNA
ENZYMES
ISOTOPES
LIGHT NUCLEI
MEMBRANE PROTEINS
MITOGENS
MUTATIONS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
550201* - Biochemistry- Tracer Techniques