Intestinal absorption of retinol and retinyl palmitate in the rat. Effects of tetrahydrolipstatin

Journal Article · Sat Sep 01 04:00:00 EDT 1990 · Lipids; (USA)

DOI:https://doi.org/10.1007/BF02537163· OSTI ID:6324343

Fernandez, E; Borgstroem, B ^[1]

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1. Univ. of Lund (Sweden)

The aim of the present study was to characterize the intestinal absorption of retinol and retinyl palmitate in thoracic duct and bile duct fistulated rats and to investigate the effect of a simultaneously administered lipase inhibitor, tetrahydrolipstatin (THL). Absorption was determined as lymphatic recovery over a 24-hr period, including an initial 12-hr continuous intraduodenal infusion of either (11,12-3H)retinol or (11,12-3H)retinyl palmitate given in emulsified glyceryl trioleate or in mixed micellar solution of monoolein and oleic acid. From micellar dispersion, labeled retinol and retinyl palmitate were recovered in the lymph to 50-60% and both to the same extent. Administered in emulsified form, labeled retinol from fed retinyl palmitate was recovered to 47%, but retinol from fed retinol to only 18%. THL (10(-4) M) in the infusate had no significant effect on the recovery of 14C-labeled oleic acid. The recovery of label from emulsified glyceryl tri(1-14C)oleate was significantly decreased at this concentration of THL (76.5% vs 19.6% recovery). When administered in emulsified form, retinol absorption was not significantly affected by THL at 10(-4) M, while retinyl palmitate absorption was very significantly decreased (5.0% compared to 47.8%). In the presence of THL, retinol absorption from retinyl palmitate in micellar solution was decreased (from 58% to 17%). Most of the retinol in the lymph extracts (72.2 to 91.3) was present as retinyl ester, regardless of the chemical and physical form of administration. Furthermore, THL did not induce any change in this pattern.

• OSTI ID: 6324343
• Journal Information: Lipids; (USA), Journal Name: Lipids; (USA) Vol. 25:9; ISSN LPDSA; ISSN 0024-4201
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ANIMALS
BILIARY TRACT
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
CARBOXYLESTERASES
CARBOXYLIC ACID ESTERS
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
ENZYME INHIBITORS
ENZYMES
ESTERASES
ESTERS
HEXADECANOIC ACID
HYDROGEN COMPOUNDS
HYDROLASES
INTESTINAL ABSORPTION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPASES
MAMMALS
MEMBRANE TRANSPORT
MONOCARBOXYLIC ACIDS
OLEIC ACID
ORGANIC ACIDS
ORGANIC COMPOUNDS
RATS
REACTION KINETICS
RETINOIC ACID
RODENTS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES