Selenite-induced variation in glutathione peroxidase activity of three mammalian cell lines: no effect on radiation-induced cell killing or DNA strand breakage

Journal Article · Wed Feb 01 04:00:00 EST 1989 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3577332· OSTI ID:6323702

Sandstroem, B E.C.; Carlsson, J; Marklund, S L

The selenium-dependent glutathione peroxidase activities of three mammalian cell lines, HT29, P31, and N-18, cultured in medium with low serum content, increased about 2-, 5-, and 40-fold, respectively, after supplementation with 100 nM selenite. Catalase, CuZn superoxide dismutase, and Mn superoxide dismutase activities were not generally influenced by selenite supplementation, and there was only a minor nonselenium-dependent glutathione peroxidase activity in the investigated cell lines. Gamma-irradiated control and selenite-supplemented cells showed no changes in the surviving fractions, as estimated by clonogenic survival or (/sup 3/H)-thymidine uptake, nor were there any significant differences between the two groups in the induction of DNA strand breaks after gamma irradiation under repairing (37 degrees C) or nonrepairing (0 degrees C) conditions. The results suggest that selenium-dependent glutathione peroxidase does not contribute significantly to the radiation resistance of cultured mammalian cells.

• Research Organization: Swedish Defense Research Establishment, Umea
• OSTI ID: 6323702
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 117:2; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560120 -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CATALASE
CELL KILLING
DNA REPAIR
DRUGS
ELECTROMAGNETIC RADIATION
ELEMENTS
ENZYME ACTIVITY
ENZYMES
GAMMA RADIATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GLUTATHIONE
IONIZING RADIATIONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MICE
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PEPTIDES
PEROXIDASES
POLYPEPTIDES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOPROTECTIVE SUBSTANCES
RADIOSENSITIVITY
RECOVERY
REPAIR
RODENTS
SELENIUM
SEMIMETALS
STRAND BREAKS
SUPEROXIDE DISMUTASE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VERTEBRATES