Multinuclear magnetic resonance studies of the 2Fe-2S sup * ferredoxin from Anabaena species strain PCC 7120. 2. Sequence-specific carbon-13 and nitrogen-15 resonance assignments of the oxidized form

Journal Article · Tue Apr 24 00:00:00 EDT 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00468a030· OSTI ID:6323192

Oh, Byung-Ha; Mooberry, E S; Markley, J L ^[1]

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1. Univ. of Wisconsin, Madison (USA)

Multinuclear two-dimensional NMR techniques were used to assign nearly all diamagnetic {sup 13}C and {sup 15}N resonances of the plant-type 2Fe{center dot}2S* ferredoxin from Anabaena sp. strain PCC 7120. Since a {sup 13}C spin system directed strategy had been used to identify the {sup 1}H spin systems, the sequence-specific {sup 1}H assignments also provided sequence-specific {sup 13}C assignments. Several resonances from {sup 1}H-{sup 13}C groups were assigned independently of the {sup 1}H assignments by considering the distances between these nuclei and the paramagnetic 2Fe{center dot}2S center. A {sup 13}C-{sup 15}N correlation data set was used to assign additional carbonyl carbons and to analyze overlapping regions of the {sup 13}C-{sup 13}C correlation spectrum. Sequence-specific assignments of backbone and side-chain nitrogens were based on {sup 1}H-{sup 15}N and {sup 13}C-{sup 15}N correlations obtained from various two-dimensional NMR experiments.

• OSTI ID: 6323192
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:16; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACID SEQUENCE
CARBON 13
CARBON ISOTOPES
CHEMICAL REACTIONS
COMPLEXES
CYANOBACTERIA
EVEN-ODD NUCLEI
FERREDOXIN
HYPERFINE STRUCTURE
IRON COMPLEXES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
METALLOPROTEINS
MICROORGANISMS
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
NITROGEN 15
NITROGEN ISOTOPES
NUCLEAR MAGNETIC RESONANCE
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OXIDATION
PROTEINS
RESONANCE
STABLE ISOTOPES
SULFUR COMPLEXES
TRANSITION ELEMENT COMPLEXES