Multinuclear magnetic resonance studies of the 2Feter dot 2S sup * ferredoxin from Anabaena species strain PCC 7120. 1. Sequence-specific hydrogen-1 resonance assignments and secondary structure in solution of the oxidized form

Journal Article · Tue Apr 24 00:00:00 EDT 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00468a029· OSTI ID:6170298

Oh, Byung-Ha; Markley, J L ^[1]

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1. Univ. of Wisconsin, Madison (USA)

Complete sequence-specific assignments were determined for the diamagnetic {sup 1}H resonances from Anabaena 7120 ferredoxin. A novel assignment procedure was followed whose first step was the identification of the {sup 13}C spin systems of the amino acids by a {sup 13}C({sup 13}C) double quantum correlation experiment. Then, the {sup 1}H spin systems of the amino acids were identified from the {sup 13}C spin systems by means of direct and relayed {sup 1}H({sup 13}C) single-bond correlations. The sequential resonance assignments were based mainly on conventional interresidue {sup 1}H{sup {alpha}}{sub i}-{sup 1}H{sup N}{sub i+1} NOE connectivities. Resonances from 18 residues were not resolved in two-dimensional {sup 1}H NMR spectra. When these residues were mapped onto the X-ray crystal structure of the homologous ferredoxin from Spirulina platensis, it was found that they correspond to amino acids close to the paramagnetic 2Fe{center dot}2S cluster. Cross peaks in two-dimensional homonuclear {sup 1}H NMR spectra were not observed for any protons closer than about 7.8 {angstrom} to both iron atoms. Secondary structural features identified in solution include two antiparallel {beta}-sheets, one parallel {beta}-sheet, and one {alpha}-helix.

• OSTI ID: 6170298
• Journal Information: Biochemistry; (USA), Journal Name: Biochemistry; (USA) Vol. 29:16; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
AMINO ACID SEQUENCE
BARYONS
CARBON 13
CARBON ISOTOPES
CHEMICAL SHIFT
COHERENT SCATTERING
COMPLEXES
CYANOBACTERIA
DIFFRACTION
ELEMENTARY PARTICLES
EVEN-ODD NUCLEI
FERMIONS
FERREDOXIN
HADRONS
IRON COMPLEXES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
METALLOPROTEINS
MICROORGANISMS
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
NUCLEI
NUCLEONS
ORGANIC COMPOUNDS
OVERHAUSER EFFECT
PROTEINS
PROTONS
RESONANCE
SCATTERING
STABLE ISOTOPES
SULFUR COMPLEXES
TRANSITION ELEMENT COMPLEXES
X-RAY DIFFRACTION