Intercalation complex of proflavine with DNA: Structure and dynamics by solid-state NMR
- State Univ. of New York, Stony Brook (USA)
The structure of the complex formed between the intercalating agent proflavine and fibrous native DNA was studied by one- and two-dimensional high-resolution solid-state nuclear magnetic resonance (NMR). Carbon-13-labeled proflavine was used to show that the drug is stacked with the aromatic ring plane perpendicular to the fiber axis and that it is essentially immobile. Natural abundance carbon-13 NMR of the DNA itself shows that proflavine binding does not change the puckering of the deoxyribose ring. However, phosphorus-31 NMR spectra show profound changes in the orientation of the phosphodiester grouping on proflavine binding, with some of the phosphodiesters tilting almost parallel to the helix axis, and a second set almost perpendicular. The first group to the phosphodiesters probably spans the intercalation sites, whereas the tilting of the second set likely compensates for the unwinding of the DNA by the intercalator.
- OSTI ID:
- 6322864
- Journal Information:
- Science (Washington, D.C.); (USA), Vol. 249:4964; ISSN 0036-8075
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
MOLECULAR STRUCTURE
PROFLAVINE
BIOCHEMICAL REACTION KINETICS
CARBON 13
CHEMICAL SHIFT
HELICAL CONFIGURATION
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
PHOSPHORUS 31
TRACER TECHNIQUES
ACRIDINES
AMINES
AROMATICS
AZAARENES
AZINES
CARBON ISOTOPES
CONFIGURATION
EVEN-ODD NUCLEI
FLAVINES
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAGNETIC RESONANCE
MUTAGENS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHORUS ISOTOPES
PYRIDINES
REACTION KINETICS
RESONANCE
SPECTRA
STABLE ISOTOPES
550201* - Biochemistry- Tracer Techniques