Metabolites of IPPA, BMIPP, and DMIPP fatty acids in rats hearts: A quantitative HPLC-study

Conference · Mon Jan 01 04:00:00 EST 1990

OSTI ID:6322089

Kropp, J; Assmann, T; Biersack, H J; Wissen, H P ^[1]

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1. Bonn Univ. (Germany, F.R.)

The significance of the use of radioiodinated fatty acid analogues such as 15-(p-iodophenyl) pentadecanoic acid (IPPA), 15-(p-iodophenyl)-3-R,S-methylpentadecanoic acid (BMIPP), and 15-(p-iodophenyl)-3-dimethylpentadecanoic acid (DMIPP) for the evaluation of regional myocardial fatty acid uptake is well documented. An understanding of the relative incorporation of these fatty acid analogues into various fatty acid pools is important to correlate fatty acid uptake and release with flux through the various metabolic pathways. While the free fatty acid (FFA) pool is immediately available for oxidation to meet energy demands for contraction under normoxic conditions, the FFA are also stored as triglycerides (TG) for oxidation at a later period. Although the incorporation of IPPA, BMIPP, and DMIPP into the major phospholipid classes has not been previously investigated, demonstration of the incorporation of IPPA and BMIPP into phospholipids would be important as further evidence that these modified'' fatty acids are, at least qualitatively, metabolized through the usual metabolic pathways. The goals of the present studies were thus to develop the necessary HPLC techniques and evaluate the incorporation of IPPA, BMIPP, and DMIPP into complex endogenous lipids in dual label studies. 2 refs., 6 figs.

• Research Organization: Oak Ridge National Lab., TN (USA)
• Sponsoring Organization: DFG; DOE/ER; DOHHS
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6322089
• Report Number(s): CONF-900529-1; ON: DE91002359; CNN: HL35500
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BIOLOGICAL PATHWAYS
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
CHROMATOGRAPHY
HALOGENATION
HEART
IN VIVO
IODINATION
LABELLED COMPOUNDS
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
METABOLITES
MONOCARBOXYLIC ACIDS
MUSCLES
MYOCARDIUM
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANS
RATS
RODENTS
SEPARATION PROCESSES
UPTAKE
VERTEBRATES