Occurrence of metallothionein gene smtA in synechococcus Tx-20 and other blue-green algae
Conference
·
· Journal of Phycology; (USA)
OSTI ID:6318444
- Univ. of Durham (England)
Blue-green algae are often abundant at Zn- and Cd-contaminated sites. In order to understand the mechanisms associated with Zn- and Cd-tolerance, we have isolated a metallothionein gene, designated smtA, in Synechococcus Tx-20 (- Pcc 6301 - Anacystis nidulans), a strain apparently obtained from an unpolluted site. The gene was cloned and sequenced, and its expression investigated in a range of heavy-metal-tolerant strains of the same organism obtained by stepwise adaptation. The polymerase chain reaction was used to probe for the possible presence of the homologous gene in a range of other strains (especially Synechococcus) isolated from sites without and with heavy metal contamination.
- OSTI ID:
- 6318444
- Report Number(s):
- CONF-9006266--
- Journal Information:
- Journal of Phycology; (USA), Journal Name: Journal of Phycology; (USA) Vol. 26:2; ISSN 0022-3646; ISSN JPYLA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550200 -- Biochemistry
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALGAE
CADMIUM
CLONING
DNA HYBRIDIZATION
DNA POLYMERASES
DNA SEQUENCING
DNA-CLONING
ELEMENTS
ENZYMES
GENES
HYBRIDIZATION
METALLOPROTEINS
METALLOTHIONEIN
METALS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
POLYMERASES
PROTEINS
SENSITIVITY
STRUCTURAL CHEMICAL ANALYSIS
TOLERANCE
TOXICITY
TRANSFERASES
ZINC
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALGAE
CADMIUM
CLONING
DNA HYBRIDIZATION
DNA POLYMERASES
DNA SEQUENCING
DNA-CLONING
ELEMENTS
ENZYMES
GENES
HYBRIDIZATION
METALLOPROTEINS
METALLOTHIONEIN
METALS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
POLYMERASES
PROTEINS
SENSITIVITY
STRUCTURAL CHEMICAL ANALYSIS
TOLERANCE
TOXICITY
TRANSFERASES
ZINC