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Coexistence of S. cerevisiae and E. coli in a chemostat under substrate competition and product inhibition

Conference ·
OSTI ID:6316173
;
A mixed culture of Saccharomyces cerevisiae and Esherichia coli was established in a stable coexistence steady state in a chemostat under constant operating conditions. The species competed for glucose, the growth limiting resource, and produced acetate and ethanol. The acetic acid was shown to be very inhibitory to E. coli in pure culture at pH 5, while ethanol inhibition was only marginal. No significant inhibition of S. cerevisiae growth was observed by either acetate or ethanol. Pure culture parameters were measured and used in the analysis. Linearized stability analysis for the case when both organisms produce the inhibitor showed that a transition through three stable outcomes was possible as the feed concentration is lowered. Experimental studies verified these predictions and successive transitions from a yeast growth steady state, to a coexistence steady state, and to a E. coli growth steady state were obtained by lowering the glucose concentration in the feed from 10 to 5 to 2.5 g/l. This dynamic behavior is distinctly different from other competition-inhibition combinations and demonstrates for the first time that coexistence is possible due to substrate competition and product inhibition. 19 refs., 13 figs., 2 tabs.
Research Organization:
Oak Ridge National Lab., TN (USA); California Inst. of Tech., Pasadena (USA). Dept. of Chemical Engineering; Massachusetts Inst. of Tech., Cambridge (USA). Dept. of Chemical Engineering
DOE Contract Number:
AC05-84OR21400
OSTI ID:
6316173
Report Number(s):
CONF-851123-10; ON: DE86003095
Country of Publication:
United States
Language:
English

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Related Subjects

09 BIOMASS FUELS
090222* -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ACETIC ACID
ALCOHOLS
ALDEHYDES
BACTERIA
BIOREACTORS
CARBOHYDRATES
CARBOXYLIC ACIDS
CULTIVATION TECHNIQUES
CULTURE MEDIA
DATA
ESCHERICHIA COLI
ETHANOL
EXPERIMENTAL DATA
FUNGI
GLUCOSE
GROWTH
HEXOSES
HYDROXY COMPOUNDS
INFORMATION
INHIBITION
KINETICS
MICROORGANISMS
MONOCARBOXYLIC ACIDS
MONOSACCHARIDES
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
YEASTS