Steady-state and transient-state analysis of growth and metabolite production in a Saccharomyces cerevisiae strain with reduced pyruvate-decarboxylase activity

Journal Article · Thu Jul 01 04:00:00 EDT 1999 · Biotechnology and Bioengineering

DOI:https://doi.org/10.1002/(SICI)1097-0290(1999)66:1<42::AID-BIT4>3.3.CO;2-C· OSTI ID:20006611

Flikweert, M T; Kuyper, M; Maris, A J.A. van; Koetter, P; Dijken, J.P. van; Pronk, J T

Pyruvate decarboxylase is a key enzyme in the production of low-molecular-weight byproducts (ethanol, acetate) in biomass-directed applications of Saccharomyces cerevisiae. To investigate whether decreased expression levels of pyruvate decarboxylase can reduce byproduct formation, the PDC2 gene, which encodes a positive regulator of pyruvate-decarboxylase synthesis, was inactivated in the prototrophic strain S. cerevisiae CEN.PK113-7D. This caused a 3--4-fold reduction of pyruvate-decarboxylase activity in glucose-limited, aerobic chemostat cultures grown at a dilution rate of 0.10 h{sup {minus}1}. Upon exposure of such cultures to a 50 mM glucose pulse, ethanol and acetate were the major byproducts formed by the wild type. In the pdc2{Delta} strain, formation of ethanol and acetate was reduced by 60--70%. In contrast to the wild type, the pdc2{Delta} strain produced substantial amounts of pyruvate after a glucose pulse. Nevertheless, its overall byproduct formation was ca. 50% lower. The specific rate of glucose consumption after a glucose pulse to pdc2{Delta} cultures was about 40% lower than in wild-type cultures.

• Research Organization: Delft Univ. of Technology (NL)
• OSTI ID: 20006611
• Journal Information: Biotechnology and Bioengineering, Journal Name: Biotechnology and Bioengineering Journal Issue: 1 Vol. 66; ISSN BIBIAU; ISSN 0006-3592
• Country of Publication: United States
• Language: English

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