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Investigating the role of the transcriptional regulator Ure2 on the metabolism of Saccharomyces cerevisiae: a multi-omics approach

Journal Article · · Applied Microbiology and Biotechnology
 [1];  [2];  [2];  [2];  [3];  [2];  [2];  [2]
  1. Univ. of Illinois at Urbana-Champaign, IL (United States). Center for Advanced Bioenergy and Bioproducts Innovation (CABBI); University of Illinois
  2. Univ. of Illinois at Urbana-Champaign, IL (United States). Center for Advanced Bioenergy and Bioproducts Innovation (CABBI)
  3. Univ. of Illinois at Urbana-Champaign, IL (United States). Center for Advanced Bioenergy and Bioproducts Innovation (CABBI); Korea Univ., Seoul, (Korea, Republic of)
+ Show Author Affiliations
Ure2 regulates nitrogen catabolite repression in Saccharomyces cerevisiae. Deletion of URE2 induces a physiological state mimicking the nitrogen starvation and autophagic responses. Previous work has shown that deletion of URE2 increases the fermentation rate of some wine-producing strains of S. cerevisiae. In this work, we investigated the effect of URE2 deletion (ΔURE2) on the metabolism of S. cerevisiae. During growth on glucose, the ΔURE2 mutant grew less well than the wild type; however, it produced ethanol at higher rates. To better under the behavior of this mutant, we performed transcriptomics and metabolomics. Analysis of the RNA sequencing results and metabolite levels indicate that the mutant strain exhibited characteristics of both nitrogen starvation and autophagy. In addition, many pyruvate decarboxylase and alcohol dehydrogenase isoforms were expressed at higher rates than the wild type. The mutant also accumulated less trehalose and glycogen, and produced more lipids. These results suggest that URE2 may be a promising target for metabolic engineering in S. cerevisiae and potentially other strains of yeast as well for the production of lipid-based fuels and chemicals.
Research Organization:
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
SC0018420
OSTI ID:
1834389
Journal Information:
Applied Microbiology and Biotechnology, Journal Name: Applied Microbiology and Biotechnology Journal Issue: 12 Vol. 105; ISSN 0175-7598
Publisher:
SpringerCopyright Statement
Country of Publication:
United States
Language:
English

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Saccharomyces cerevisiae
URE2
ethanol
glycogen
lipid
metabolomics
transcriptomics
trehalose