Antibody analysis of relationships among methanogenic bacteria

Journal Article · Fri Jan 01 04:00:00 EST 1982 · J. Bacteriol.; (United States)

OSTI ID:6310312

Conway de Macario, E; Wolin, M J; Macario, A J.L.

A bank of antisera to the majority of methanogenic bacteria is now available. Three antibody probes, R, S, and T, were derived from each antiserum in the bank and used for analysis of antigenic relatedness among methanogens by immunofluorescence. The T probe reacted only with the immunizing (or homologous) strain, the S probe gave strong cross-reactions with strains of the same species, and the R probe revealed some interspecies relationships. The results were confirmed and extended by enzyme immunoassays and standard serological methods involving serial dilution analysis, cross-adsorptions, and the use of reference strains. The immunological methods and standardized antibody probes are useful for rapid identification of methanogens and measurements of antigenic relationships which aid in the classification of these bacteria.

• Research Organization: New York State Dept. of Health, Albany
• DOE Contract Number: AC02-81ER10880
• OSTI ID: 6310312
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 149:1; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ANTIGEN-ANTIBODY REACTIONS
BACTERIA
BIOASSAY
CLASSIFICATION
ENZYME ACTIVITY
FLUORESCENCE
IMMUNE SERUMS
LUMINESCENCE
METHANOGENIC BACTERIA
MICROORGANISMS