Specific antisera and immunological procedures for characterization of methanogenic bacteria
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:6180655
Specific antisera were raised in rabbits to 19 methanogenic bacteria representing the species available in pure culture at the present time. The antisera were characterized, labeled, and organized in a bank to serve as a source of material for preparation of antibody probes and thus provide standardized reagents for immunological analysis of methanogens. An indirect immunofluorescence procedure was standardized for optimal staining of homologous and heterologous bacterial strains. Two immunoenzymatic assays were developed. Procedural details were developed to obtain optimal bacterial preparations for use as immunogens to raise antibodies in vivo, and as antigens for antibody assay in vitro.
- Research Organization:
- New York State Dept. of Health, Albany
- DOE Contract Number:
- AC02-81ER10880
- OSTI ID:
- 6180655
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 149:1; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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