Attempted induction of resistance to melphalan by pretreating Chinese hamster cells with subtoxic doses of the drug

Technical Report · Sat Sep 01 00:00:00 EDT 1984

OSTI ID:6309445

Kaye, M F; Tobey, R A

By pretreating the line Chinese hamster (CHO) cells with multiple subtoxic doses of melphalan before challenge with a toxic dose, we established optimal conditions to increase the resistance of the cells to melphalan toxicity. Variables examined included: (1) level of drug used to induce protection (0.0008 to 0.8 ..mu..M); (2) interval between administration of successive low doses of drug (3 to 12 h); and (3) duration of induction period (24 to 96 h). The greatest degree of protection (an increase by 2 to 2.5 times in survival following challenge with 16 ..mu..M melphalan for 2 h) was achieved with a level of 0.08 ..mu..M melphalan administered every 6 h for 48 h. Combining the low-level drug induction protocol described above with our previously described trace element induction protocol (treatment with 125 ..mu..M CuCl/sub 2/ for the last 9 h of the induction period) increased survival slightly over that achieved with either single-induction protocol. If we assume that the induction protocols yielded a slight but real degree of protection, it appears that the induced protection comprises a common component representing activities elicited by the two induction protocols and a component that represents protective processes that are unique to a given induction protocol. 10 references, 1 figure, 3 tables.

• Research Organization: Los Alamos National Lab., NM (USA)
• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 6309445
• Report Number(s): LA-10176-MS; ON: DE85002342
• Resource Relation: Other Information: Portions are illegible in microfiche products. Original copy available until stock is exhausted
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKYLATING AGENTS
TOXICITY
CHO CELLS
SENSITIVITY
ANTINEOPLASTIC DRUGS
EXPERIMENTAL DATA
RESPONSE MODIFYING FACTORS
ANIMAL CELLS
DATA
DRUGS
INFORMATION
NUMERICAL DATA
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)