Effects of cadmium on karyotype stability in Chinese hamster ovary cells. Progress report, July 1, 1976--June 30, 1977

Technical Report · Sat Oct 01 04:00:00 EDT 1977

DOI:https://doi.org/10.2172/5265741· OSTI ID:5265741

Deaven, L L; Campbell, E W

Chinese hamster cells (line CHO) were examined for cadmium-induced growth rate perturbations and chromosome aberrations. Experimental protocols were designed to simulate conditions of cellular Cd/sup + +/ exposure both in vivo and in vitro. CHO cells grown in the presence of nontoxic levels of Cd/sup + +/ (2 x 10/sup -7/ M) for 12 wk became resistant to toxic levels (2 x 10/sup -6/ M) after this exposure. Metaphase cells in these resistant populations contained only background levels of chromosome aberrations. This suggests that individuals exposed to constant or intermittent low levels of Cd/sup + +/ may be more resistant to the toxic effects of this metal and may not show chromosome aberrations as readily as nonexposed individuals. CHO cells grown in medium supplemented with several types of serum (fetal calf, newborn calf, and human) at concentrations commonly used for in vitro culture (5 to 20 percent v/v) or found in mammalian circulatory systems (50 percent v/v) differed markedly in Cd/sup + +/ tolerance. Cells grown in medium containing 1 x 10/sup -6/ M Cd/sup + +/ and supplemented with human or newborn calf serum were slightly protected against Cd/sup + +/ damage at high serum concentrations (30 and 50 percent v/v) and accumulated approximately 90 ..mu..g Cd/sup + +//10/sup 9/ cells in 48 h. Cells growing at low or high concentrations of fetal calf serum were completely protected from 1 x 10/sup -6/ M Cd/sup + +/ and accumulated approximately 12 ..mu..g Cd/sup + +//10/sup 9/ cells in 48 h. These results demonstrate the necessity for standardized protocols for cytogenetic investigations of Cd/sup + +/ toxicity and should help to explain discrepancies between studies of chromosome damage in patients with high blood levels of cadmium.

• Research Organization: Los Alamos Scientific Lab., N.Mex. (USA)
• DOE Contract Number: W-7405-ENG-36
• OSTI ID: 5265741
• Report Number(s): LA-6983-PR
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BLOOD SERUM
CADMIUM
CHROMOSOMAL ABERRATIONS
CULTURE MEDIA
ELEMENTS
HAMSTERS
MAMMALS
METALS
MUTATIONS
PATIENTS
RODENTS
TOXICITY
VERTEBRATES