Effect of colchicine on rat small intestinal absorptive cells. II. Distribution of label after incorporation of (/sup 3/H)fucose into plasma membrane glycoproteins

Journal Article · Wed Nov 30 23:00:00 EST 1983 · J. Ultrastruct. Res.; (United States)

DOI:https://doi.org/10.1016/S0022-5320(83)90038-2· OSTI ID:6307433

Ellinger, A; Pavelka, M; Gangl, A

By means of radioautography the influence was tested of various periods (5, 15, 30, 40 min, 2 hr) of pretreatment with colchicine, administered intraperitoneally to rats at a dosage of 0.5 mg/100 g of body weight, on the intracellular pathway of (/sup 3/H)fucose in absorptive cells of the small intestine. Administration of colchicine for 30 min and longer time intervals causes delay in the insertion of (/sup 3/H)fucose into the oligosaccharide chains of glycoconjugates in the Golgi apparatus, and results in redistribution of the label apparent over the different portions of the plasma membrane. In controls, at 2 and 4 hr after administration of (/sup 3/H)fucose the apical plasma membrane is strongly labeled. Colchicine causes equalization of the reaction of apical and basolateral regions of the plasma membrane: the number of silver grains attributable to the apical plasma membrane is reduced; following treatment with colchicine, apical portions of the plasma membrane comprise 31.6 +/- 1.8% of the silver grains, 38.6 +/- 3.8% are attributable to basolateral membrane regions. The colchicine-induced equalization of the density of label of apical and basolateral regions of the plasma membrane, in addition to the occurrence of basolateral microvillus borders, suggests microtubules to be important in the maintenance of the polar organization of small intestinal absorptive cells.

• Research Organization: Univ. of Vienna, Austria
• OSTI ID: 6307433
• Journal Information: J. Ultrastruct. Res.; (United States), Journal Name: J. Ultrastruct. Res.; (United States) Vol. 85:3; ISSN JULRA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ABSORPTION
ALKALOIDS
ANIMALS
ANTIMITOTIC DRUGS
ANTIPYRETICS
AUTORADIOGRAPHY
BIOCHEMISTRY
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHEMISTRY
COLCHICINE
DIGESTIVE SYSTEM
DRUGS
GASTROINTESTINAL TRACT
GLUCOPROTEINS
HEXOSES
INTESTINAL ABSORPTION
INTESTINES
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
MICROTUBULES
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RATS
RODENTS
SACCHARIDES
SMALL INTESTINE
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES