Influence of colchicine and vinblastine on the intracellular migration of secretory and membrane glycoproteins: III. Inhibition of intracellular migration of membrane glycoproteins in rat intestinal columnar cells and hepatocytes as visualized by light and electron-microscope radioautography after 3H-fucose injection
In the present work, the effects of these drugs on migration of membrane glycoproteins have been examined at the ultrastructural level in duodenal villous columnar cells and hepatocytes. Young (40 gm) rats were given a single intravenous injection of colchicine (4.0 mg) or vinblastine (2.0 mg). At 10 min after colchicine and 30 min after vinblastine administration, the rats were injected with 3H-fucose. Control rats received 3H-fucose only. All rats were sacrificed 90 min after 3H-fucose injection and their tissues processed for radioautography. In duodenal villous columnar cells, 3H-fucose labeling of the apical plasma membrane was reduced by 51% after colchicine and by 67% after vinblastine treatment; but there was little change in labeling of the lateral plasma membrane. Labeling of the Golgi apparatus increased. This suggests that labeled glycoproteins destined for the apical plasma membrane were inhibited from leaving the Golgi region, while migration to the lateral plasma membrane was not impaired. In hepatocytes, labeling of the sinusoidal plasma membrane was reduced by 83% after colchicine and by 85% after vinblastine treatment. Labeling of the lateral plasma membrane also decreased, although not so dramatically. Labeling of the Golgi apparatus and neighboring secretory vesicles increased. This indicates that the drugs inhibited migration of membrane glycoproteins from the Golgi region to the various portions of the plasma membrane. Accumulation of secretory vesicles at the sinusoidal front suggests that exocytosis may also have been partially inhibited. In both cell types, microtubules almost completely disappeared after drug treatment. Microtubules may, therefore, be necessary for intracellular transport of membrane glycoproteins, although the possibility of a direct action of these drugs on Golgi or plasma membranes must also be considered.
- Research Organization:
- McGill Univ., Montreal, Quebec, Canada
- OSTI ID:
- 6241597
- Journal Information:
- Am. J. Anat.; (United States), Journal Name: Am. J. Anat.; (United States) Vol. 170:4; ISSN AJANA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ALKALOIDS
ANIMAL CELLS
ANTIMITOTIC DRUGS
ANTIPYRETICS
AROMATICS
AUTORADIOGRAPHY
AZAARENES
AZOLES
BIOLOGICAL EFFECTS
BODY
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM DEPRESSANTS
COLCHICINE
DIGESTIVE SYSTEM
DRUGS
GASTROINTESTINAL TRACT
GLANDS
GLUCOPROTEINS
HETEROCYCLIC COMPOUNDS
HEXOSES
INDOLES
INTESTINES
LABELLED COMPOUNDS
LIVER
MEMBRANE TRANSPORT
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRROLES
SACCHARIDES
SOMATIC CELLS
TRITIUM COMPOUNDS
VINBLASTINE