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Synaptic input to vasopressin neurons of the paraventricular nucleus (PVN)

Journal Article · · Peptides (Fayetteville, N.Y.); (United States)
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Following injections of horseradish peroxidase into the PVN, retrogradely filled cells were found in regions of the limbic system known to contain glucocorticoid concentrating neurons. To determine if these regions which include the lateral septum, medial amygdala and ventral subiculum have a monosynaptic input to vasopressin neurons the authors developed a double label ultrastructural technique to simultaneously visualize immunoreactive neuropeptide and anterogradely transported HRP. Following injections of tracer into all three of these regions, HRP labeled fibers were seen at the light microscopic level to form a halo in the perinuclear, cell poor zone around the PVN. Ultrastructural examination of this area resulted in the discovery of a small number of limbic system synapses on vasopressin dendrites. In a similar fashion they were interested in determining the distribution of noradrenergic terminals on vasopressin neurons in the various subnuclei of the PVN. The authors have combined immunocytochemistry for vasopressin with radioautography for /sup 3/H-norepinephrine (NE) at the ultrastructural level. NE terminals were numerous in the periventricular zone, innervating both vasopressin containing dendrites and non-immunoreactive dendrites and cell bodies. These studies demonstrate the need for ultrastructural analysis of synaptic input to neurosecretory cells.

Research Organization:
Columbia Univ. Coll. of Physicians and Surgeons, New York, NY
OSTI ID:
6306276
Journal Information:
Peptides (Fayetteville, N.Y.); (United States), Journal Name: Peptides (Fayetteville, N.Y.); (United States) Vol. 5 Suppl 1; ISSN PPTDD
Country of Publication:
United States
Language:
English

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Related Subjects

550801* -- Morphology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BIOCHEMISTRY
BODY
BRAIN
CARBOXYLIC ACIDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
CHEMISTRY
DRUGS
ELECTRON MICROSCOPY
ENZYMES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HORMONES
LABELLED COMPOUNDS
MAMMALS
MICROSCOPY
MORPHOLOGICAL CHANGES
NERVE CELLS
NERVOUS SYSTEM
NEUROREGULATORS
NORADRENALINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEPTIDE HORMONES
PEROXIDASES
PITUITARY HORMONES
PORPHYRINS
RATS
RODENTS
SOMATIC CELLS
STEROID HORMONES
SYMPATHOMIMETICS
TRITIUM COMPOUNDS
ULTRASTRUCTURAL CHANGES
VASOPRESSIN
VERTEBRATES